letnaMon-Fri 07:30-19:00, Sat-Sun 09:00-19:00Walk-in onlySourdough, kolache, breakfast pastry
Eska Letna opened in February 2025 on the Letna grid as the second Ambiente bakery, baking the same bread 33 sourdough plus laminated pastries and breakfast to-go.
Tip: Sweet and savoury breakfasts are made to-go; the morning crowd is the Letna runners.
Worth the queue: Bread 33
karlinMon-Fri 07:00-19:00, Sat-Sun 08:00-18:00Walk-in onlyWheat-rye breads, potato sourdough, chlebicky
Antoninovo Pekarstvi opened the first of its six city-wide bakeries in Karlin in 2014. Wheat-rye and potato sourdough loaves, all baked on-site, plus made-up chlebicky and soups.
Tip: The decorated chlebicky are made up at the counter; ask for a flight to-go.
Worth the queue: Potato sourdough loaf
vinohradyTue-Sat 07:30-18:00Walk-in onlyStone-milled sourdough breads, heritage grains
Pekarna Praktika is Tomas Solak's serious sourdough bakery on the Vinohrady-Vrsovice border. Solak mills his own flour on-site and works with farmers growing heritage grains.
Tip: The pain de campagne goes fast; freezer-ready loaves stay good a week.
Worth the queue: Stone-milled wheat sourdough
nove-mestoMon-Fri 07:30-19:00, Sat-Sun 08:00-18:00Walk-in onlyFrench laminated pastry, sourdough baguette
Petite France on Myslikova is the city's most committed French bakery, behind a mint-green shopfront in Nove Mesto. Baguettes, pain au chocolat, tarte au citron, all daily.
Tip: The croissant is the city benchmark; aim to be there by 11:00 weekends.
Worth the queue: Pain au chocolat
nove-mestoMon-Fri 06:30-18:30, Sat 07:00-12:30Walk-in onlyCzech rye breads, classic pastry
Pekarstvi Moravec is a family-run Czech bakery with multiple Prague counters. The Nove Mesto branch on Biskupska keeps to the working repertoire of rye loaves and classic Czech pastry.
Tip: The Sumava rye is the loaf to take home; closes Sunday.
Worth the queue: Sumavsky rye loaf
vinohradyMon-Fri 07:00-19:30, Sat-Sun 08:00-18:00Walk-in onlySourdough, kolache, sweet buns
Antoninovo Pekarstvi's Namesti Miru flagship opened in a restored First Republic cafe space. Breads, decorated chlebicky, lemonades and a proper sit-down corner for breakfast.
Tip: The big window seats are the prize; show up before 10:00 on weekends.
Worth the queue: Wheat-rye sourdough loaf